Human IL-5 (Research Grade Mepolizumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11433

Recombinant Monoclonal Antibody For Research Use Only.
R&D Systems, part of Bio-Techne
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MAB11433-100
MAB11433-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu212

View all IL-5 Antibodies »

Product Specifications

Immunogen

Human IL-5

Specificity

Detects human IL-5 based on Mepolizumab therapeutic antibody.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Human IL-5 (Research Grade Mepolizumab Biosimilar) Antibody

Human IL-5 ELISA Standard Curve

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human IL-5 (Research Grade Mepolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB11433) to IL-5. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.

Applications for Human IL-5 (Research Grade Mepolizumab Biosimilar) Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody for the specific antigen in direct ELISA. Colorimetric detection is performed after addition of a suitable substrate.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Mepolizumab is a humanized IgG1 k anti-IL5 monoclonal antibody. IL-5 is a key cytokine required for the maturation, activation, proliferation and survival of eosinophils making it an ideal target for therapeutic management of eosinophil levels in respiratory indications. Mechanistically, mepolizumab exerts its therapeutic function as an IL-5 antagonist to reduce eosinophil levels in the blood. IL-5 is 134-amino acid 52 kDa homodimeric protein with a four-helix domain. Mepolizumab specifically binds to the alpha-chain of IL-5, resulting in two IL-5 dimers being cross-linked by two molecules of mepolizumab. The highly specific binding of mepolizumab to IL-15 blocks ligation to IL-5Aa receptor. The biologic is primarily used for the management of moderate to severe asthma. Bio-Techne's Leronlimab biosimilar is produced using the full-length sequence which is identical to that of the original therapeutic antibody.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Human IL-5 (Research Grade Mepolizumab Biosimilar) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-5 (Research Grade Mepolizumab Biosimilar) Antibody

For research use only

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