Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 623153

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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
Gln19-Ile144
Accession # P15248

Specificity

Detects human IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat IL-9 or recombinant mouse IL-9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-9 APC-conjugated Antibody

Detection of IL‑9 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 APC-conjugated Monoclonal Antibody (Catalog # IC209A) or (B) Mouse IgG2B Isotype Control Antibody (IC0041A) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human IL-9 APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with plate-bound anti-CD3 and anti-CD28 antibodies, recombinant human IL-4, and anti-human IFN-gamma antibody were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-9

Interleukin-9 (IL-9) is a 28-30 kDa monomeric glycosylated polypeptide that belongs to the IL-7/IL-9 family of proteins. Although multiple cell types are reported to express IL-9, including Th17 cells, eosinophils, basophils, NK T cells and mast cells, the two cell types possibly most central to the process are Th9 and ILC-2 (or innate lymphoid cell type 2) cells. IL-9 supports the growth of multiple hematopoietic cell types including germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. It also promotes IgE production, the activation and chemoattraction of mast cells, mucus production by epithelium, Th17 cell proliferation and TGF-beta production by dendritic cells. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

UniProt

P15248

Additional IL-9 Products

Product Documents for Human IL-9 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-9 APC-conjugated Antibody

For research use only

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