Detects human ILT11/LILRA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human ILT11/LILRA5 PE-conjugated Antibody

Detection of ILT11/LILRA5 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3832C) and either (A) Mouse Anti-Human ILT11/LILRA5 PE-conjugated Monoclonal Antibody (Catalog # FAB6754P) or (B) Mouse IgG_2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ILT11/LILRA5 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA5/CD85f

ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-a and IL-1b. Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. ILT11 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Alternate Names

CD85f, ILT11, LILRB7, LIR9

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Gene Symbol

LILRA5

UniProt

A6NI73 (Human)

Additional LILRA5/CD85f Products

Product Documents for Human ILT11/LILRA5 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ILT11/LILRA5 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human ILT11/LILRA5 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6754P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LILRA5/CD85f Antibodies »

Product Specifications

Immunogen

Specificity