Human Integrin alpha11 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4235
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha11
Phe23-Pro1141 (Leu524Arg)
Accession # NP_001004439
Phe23-Pro1141 (Leu524Arg)
Accession # NP_001004439
Specificity
Detects human Integrin alpha11 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin alpha11 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin alpha1 and rhIntegrin alpha2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Integrin alpha11 Antibody
Detection of Human Integrin alpha11 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4235) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin a11 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Integrin alpha11 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line
Sample: A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 11
Integrin alpha11 (ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta1 integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II. ITGA11 is expressed by embryonic mesenchymal cells in and around areas that incorporate, or utilize collagen during development. In adult tissue, fibroblasts express ITGA11, and collagen:ITGA11 interaction likely contributes to wound contraction and closure. Mature human ITGA11 is an 1167 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1120 aa extracellular domain (ECD) (aa 23-1142) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta-propellers (aa 24-650) with an intervening I domain that binds collagen (aa 154-345). Over aa 23-1142, human ITGA11 shares 90% aa identity with mouse ITGA11.
Alternate Names
ITGA11
Gene Symbol
ITGA11
UniProt
Additional Integrin alpha 11 Products
Product Documents for Human Integrin alpha11 Antibody
Product Specific Notices for Human Integrin alpha11 Antibody
For research use only
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