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Human Integrin  alpha2b beta3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB76161

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2530A

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived heterodimer of Human Integrin alpha2b (Leu32-Arg993, Accession P08514) and Human Integrin beta3 (Gly27-Asp718, Accession P05106)

Specificity

Detects Human Integrin alpha2b beta3 heterodimer in direct ELISAs. In direct ELISAs, no cross-reactivity with recomabint human (rh) Integrin alpha2b, rhIntegrin alpha5, rhIntegrin alpha5 beta6, rhIntegrin alpha8 beta1, rhIntegrin beta1, rhIntegrin beta2, rhIntegrin beta3, rhIntegrin beta5, rhIntegrin beta7, and recombinant mouse Integrin alpha2b beta3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Integrin  alpha2b beta3 Antibody

Detection of Integrin a2b beta3 antibody in Human peripheral blood platelets antibody by Flow Cytometry.

Detection of Integrin alpha2b beta3 in Human peripheral blood platelets by Flow Cytometry.

Human peripheral blood platelets were stained with (A) Rabbit Anti-Human Integrin a2b beta3 Monoclonal Antibody (Catalog # MAB76161) or (B) Rabbit IgG isotype control antibody (Catalog # MAB1050) followed by Anti-Rabbit IgG APC-conjugated Secondary Antibody (Catalog # F0111) and Mouse anti-Human CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha2b beta3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood platelets

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2b beta 3

Integrin alpha2b beta3 (also alphaIIb beta3 or GPIIbIIIa) is the only alpha2b integrin and shares the beta3 subunit only with alphaV beta3 (1‑3). It is the non‑covalent heterodimer of type I transmembrane subunits, alpha2b/CD41 (present as a disulfide‑linked complex of 114 kDa heavy and 22 kDa light chains) and 93 kDa beta3/CD61 (1‑3). It is the most abundant integrin expressed by megakaryocytes and platelets, both on the surface and within alpha granules (1, 2). Deficiencies of alpha2b beta3 produce Glanzmann thrombasthenia, a potentially serious bleeding disorder (4). In its constitutively inactive state, alpha2b beta3 is flexed within the extracellular domains. Activation, either by intracellular signaling or by Mg2+ or Mn2+ binding, extends the integrin to expose the ligand binding site created by interaction of the beta3 vWFA domain with the alpha2b  beta-propeller structure (1). The 962 aa human alpha2b ECD shares 78‑83% aa sequence identity with mouse, rat, canine, equine and porcine  alpha2b while the 685 aa human  beta3 ECD shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and porcine beta3. It is unclear whether splice variants of beta3 that differ in the cytoplasmic domain are expressed significantly in platelets (5‑7). However, platelet expression of a beta3 splice variant that produces a soluble 60 kDa beta3 isoform, and an alpha2b isoform lacking aa 948‑982, have been reported (7, 8). Active cell surface alpha2b beta3 adheres to fibrinogen, mediating platelet/platelet interactions that initiate a cascade of platelet activation and aggregation, extracellular matrix adhesion, formation of thrombi and clot retraction (1). It also binds matrix proteins that have an RGD motif, including fibronectin, plasminogen, prothrombin, thrombospondin and vitronectin (1, 2). Targeting of alpha2b beta3 by therapeutic antibodies or small molecules can inhibit formation of thrombi in patients with acute coronary syndrome, and potentially inhibits tumor angiogenesis and metastasis by blocking interaction of platelet alpha2b beta3 with tumor cells (1, 9).

References

  1. Kasirer-Friede, A. et al. (2007) Immunol. Rev. 218:247.
  2. Poncz, M. et al. (1987) J. Biol. Chem. 262:8476.
  3. Fitzgerald, L.A. et al. (1987) J. Biol. Chem. 262:3936.
  4. Franchini, M. et al. (2010) Clin. Chim. Acta 411:1.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. van Kuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
  8. Bray, P.F. et al. (1990) J. Biol. Chem. 265:9587.
  9. Erpenbeck, L. and M.P. Schon (2010) Blood 115:3427.

Alternate Names

BDPLT16;BDPLT2;CD41;CD41B;GP2B;GPIIb;GT;GT1;GTA;HPA3;Integrin alpha-IIb;ITGA2B;PPP1R93

Entrez Gene IDs

3674 (Human); 16399 (Mouse); 29350 (Rat)

Gene Symbol

ITGA2B

Additional Integrin alpha 2b beta 3 Products

Product Documents for Human Integrin  alpha2b beta3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  alpha2b beta3 Antibody

For research use only

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