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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation

Cited:

ELISA Detection, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MP4F10

Product Specifications

Immunogen

SW1222 human colorectal carcinoma cell line

Specificity

Detects human Integrin  alpha6/CD49f.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Integrin  alpha6/CD49f Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunohistochemistry

Anbazhagan, R. et al. (1995) J. Pathology 176:227.

Immunoprecipitation

Anbazhagan, R. et al. (1995) J. Pathology 176:227.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 6/CD49f

The Integrin alpha6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta1 (CD29) or beta4 (CD104) subunits. Integrin alpha6 beta1 is a receptor for laminin. Integrin alpha6 beta4 is also a receptor for laminin and is a structural component of hemidesmosomes in stratified epithelia.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Gene Symbol

ITGA6

Additional Integrin alpha 6/CD49f Products

Product Documents for Human Integrin  alpha6/CD49f Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  alpha6/CD49f Antibody

For research use only

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