Human Integrin alphaV/CD51 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1219P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # P2W7
Product Specifications
Immunogen
Ocular melanoma cell line
Specificity
Detects the ectodomain of human Integrin alphaV/CD51.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin alphaV/CD51 PE-conjugated Antibody
Detection of Integrin alphaV/CD51 in HT1080 Human Cell Line by Flow Cytometry.
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 PE-conjugated Monoclonal Antibody (Catalog # FAB1219P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin alphaV/CD51 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HT1080 human fibrosarcoma cell line
Sample: HT1080 human fibrosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using FAB1219P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V/CD51
Integrin alphaV (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha-chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta-chains, including beta1, 3, 5, 6 and 8. Human alphaV is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta‑propellor domain (aa 46-483). Furin cleavage of the alphaV ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alphaV chain. alphaV-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alphaV is 92% aa identical to mouse alphaV ECD.
Alternate Names
CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA
Gene Symbol
ITGAV
Additional Integrin alpha V/CD51 Products
Product Documents for Human Integrin alphaV/CD51 PE-conjugated Antibody
Product Specific Notices for Human Integrin alphaV/CD51 PE-conjugated Antibody
For research use only
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