Human Integrin alphaV beta3 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3050A
Key Product Details
Species Reactivity
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Cited:
Applications
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Cited:
Label
Antibody Source
Product Specifications
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Integrin alphaV beta3 APC-conjugated Antibody
Detection of Integrin alphaV beta3 in HUVEC Human Cells by Flow Cytometry.
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin aV beta3 APC-conjugated Monoclonal Antibody (Catalog # FAB3050A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin alphaV beta3 APC-conjugated Antibody
Flow Cytometry
Sample: HUVEC human umbilical vein endothelial cells
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V beta 3
Integrin alphaV beta3 together with alphaIIb beta3, constitutes the only known beta3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alphaV/CD51 and 93 kDa beta3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alphaV beta3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alphaV beta3 is in the N-terminal head region, formed by interaction of the beta3 vWFA domain with the alphaV beta-propeller structure (4). The alphaV subunit contributes a thigh and a calf region, while the beta3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alphaV subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alphaV beta3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alphaV beta3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alphaV beta3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alphaV beta3 (4, 10). The 962 aa human alphaV ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow alphaV while the 685 aa human beta3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta3.
References
- Hynes, R. O. (2002) Cell 110:673.
- Serini, G. et al. (2006) Exp. Cell Res. 312:651.
- Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
- Xiong, J. et al. (2001) Science 294:339.
- Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
- vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
- Djaffar, I. et al. (1994) Biochem. J. 300:67.
- McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
- Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
- Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
- Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
- Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional Integrin alpha V beta 3 Products
Product Documents for Human Integrin alphaV beta3 APC-conjugated Antibody
Product Specific Notices for Human Integrin alphaV beta3 APC-conjugated Antibody
For research use only