Detects human Integrin beta3/CD61 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin beta1, beta2, recombinant mouse Integrin beta4, or beta6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Integrin beta3/CD61 Antibody

Detection of Integrin beta3/CD61 in PBMC monocytes by Flow Cytometry

PBMC monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Integrin beta3/CD61 Monoclonal Antibody (Catalog # MAB2266) or (B) isotype control antibody (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin beta3/CD61 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin beta3/CD61 under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB2266 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 3/CD61

Integrin beta3, also called CD61, associates with the Integrin alphaIIb or alphaV subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alphaIIb/ beta3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alphaV/ beta3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Entrez Gene IDs

3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)

Gene Symbol

ITGB3

UniProt

P05106

Additional Integrin beta 3/CD61 Products

Product Documents for Human Integrin beta3/CD61 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin beta3/CD61 Antibody

For research use only

Citations
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Calculators
Flow Cytometry