Human Integrin beta5 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3824
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta5
Gly24-Asn719
Accession # P18084
Gly24-Asn719
Accession # P18084
Specificity
Detects human Integrin beta5 in Western blots. In Western blots, less than 5% cross-reactivity with Integrin beta1, -beta 2, -beta 3, recombinant mouse Integrin beta4, -beta 6, and -beta 7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta5 Biotinylated Antibody
Integrin beta5 in MCF‑7 Human Cell Line.
Integrin beta5 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Integrin beta5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to transmembrane proteins. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin beta5 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: MG-63 human osteosarcoma cell line
Sample: MG-63 human osteosarcoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line and immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MG-63 human osteosarcoma cell line and immersion fixed MCF-7 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin beta5
Sample: Recombinant Human Integrin beta5
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 5
Integrin beta5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alphaV. Integrin alphaV beta5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta5, respectively.
Alternate Names
ITGB5
Gene Symbol
ITGB5
UniProt
Additional Integrin beta 5 Products
Product Documents for Human Integrin beta5 Biotinylated Antibody
Product Specific Notices for Human Integrin beta5 Biotinylated Antibody
For research use only
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