Human IQGAP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5068
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IQGAP2
Asn444-Ser633
Accession # Q13576
Asn444-Ser633
Accession # Q13576
Specificity
Detects endogenous human IQGAP2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human IQGAP2 Antibody
Detection of Human IQGAP2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP2 at approximately 185 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human IQGAP2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IQGAP2
IQGAP2 (IQ motif with Ras GAP-related domain 2) is a 180‑190 kDa member of the IQGAP family of proteins. It has limited expression (hepatocytes, platelets, parietal cells), binds to GTP-bound Cdc42, blocking GTP hydrolysis, and participates in actin cross-linking. Human IQGAP2 is 1575 amino acids (aa) in length. It contains one actin-binding CH domain (aa 41‑156), a Pro-binding WWP region (aa 594‑627), three consecutive calmodulin-binding IQ motifs (aa 690‑779), and a noncatalytic Ras-GAP domain (aa 917‑1150). There are multiple IQGAP2 splice variants. One shows a seven aa substitution for aa 1‑454 coupled to a deletion of aa 642‑698, while a second shows a 67 aa substitution for aa 303 and 304. IQGAP-2 is 59% aa identical to IQGAP1.
Long Name
IQ Motif Containing GTPase Activating Protein 2
Alternate Names
IQ motif containing GTPase activating protein 2, ras GTPase-activating-like protein IQGAP2
Gene Symbol
IQGAP2
UniProt
Additional IQGAP2 Products
Product Documents for Human IQGAP2 Antibody
Product Specific Notices for Human IQGAP2 Antibody
For research use only
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