Human IRF2BP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6800
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IRF2BP1
Ala188-Glu356
Accession # Q8IU81
Ala188-Glu356
Accession # Q8IU81
Specificity
Detects human IRF2BP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human IRF2BP1 Antibody
Detection of Human IRF2BP1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 hours. Gels were loaded with 30 µg of whole cell lysate (WCL) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF2BP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IRF2BP1 in 293T Human Cell Line.
IRF2BP1 was detected in immersion fixed 293T human embryonic kidney cell line using Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human IRF2BP1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed 293T human embryonic kidney cell line
Sample: Immersion fixed 293T human embryonic kidney cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line, untreated, and HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human TGF‑ beta1 (Catalog # 240-B)
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line, untreated, and HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human TGF‑ beta1 (Catalog # 240-B)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF2BP1
IRF2BP1 (Interferon regulatory factor 2 binding protein 1) is a 64-66 kDa member of the IFR2BP family of proteins. It is widely expressed, and known to bind to the C-terminus of IRF-2, creating a transcriptional repressor complex. IRF2BP1 also bind to JDP2 (Jun dimerization protein 2), promoting its ubiquitination. Human IRF2BP1 is 584 amino acids (aa) in length. It contains two Zn-finger domains (aa 6-67 and 498-584), one coiled-coil region (aa 197-217), a Cys-rich region (aa 503‑550), an N-terminal acetylation site at Ala2, and multiple utilized phosphorylation sites at Ser 66/125/186/384/421/436/453/457. Over aa 188-356, human and mouse IRF2BP1 are identical in amino acid sequence.
Long Name
Interferon Regulatory Factor 2 Binding Protein 1
Alternate Names
DKFZP434M154
Gene Symbol
IRF2BP1
UniProt
Additional IRF2BP1 Products
Product Documents for Human IRF2BP1 Antibody
Product Specific Notices for Human IRF2BP1 Antibody
For research use only
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