Human IRF3 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4019A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 482205
Product Specifications
Immunogen
E. coli-derived recombinant human IRF3
aa 206-427
Accession # Q14653
aa 206-427
Accession # Q14653
Specificity
Detects human IRF3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IRF3 APC-conjugated Antibody
Detection of IRF3 in Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IRF3 APC-conjugated Monoclonal Antibody (Catalog # IC4019A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IRF3 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Daudi human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Daudi human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IRF3
IRF3 (interferon response factor 3) is a 60 kDa member of the IRF family of proteins. Human IRF3 contains one DNA binding domain (aa 7‑107), a nuclear export signal (aa 139‑149) and multiple phosphorylation sites (aa 395‑407). Viral infection stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. Alternate splice forms may exist. One will show a deletion of aa 201‑327, a second will show the same deletion plus an alternate start site at Met147, and a third will show a 125 aa substitution for the C-terminal 100 aa (aa 328‑427). Over aa 206‑427, human IRF3 is 76% and 83% aa identical to mouse and pig IRF3, respectively.
Long Name
Interferon Regulatory Factor 3
Alternate Names
interferon regulatory factor 3, IRF-3
Gene Symbol
IRF3
UniProt
Additional IRF3 Products
Product Documents for Human IRF3 APC-conjugated Antibody
Product Specific Notices for Human IRF3 APC-conjugated Antibody
For research use only
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