Human ISG15/UCRP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4845
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Capture
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ISG15
Gly2-Gly157
Accession # ABW03539
Gly2-Gly157
Accession # ABW03539
Specificity
Detects endogenous human ISG15 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ISG15/UCRP Antibody
Detection of Human ISG15/UCRP by Western Blot.
Western blot shows lysates of DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human ISG15/UCRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4845) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human ISG15/UCRP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line
Sample: DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ISG15/UCRP
ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation, instead, it is part of the anti‑virus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2‑78 and 79‑157) and a propeptide (aa 15‑165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.
Long Name
ISG15 Ubiquitin-like Modifier
Alternate Names
G1P2, IFI15, IP17, UCRP
Gene Symbol
ISG15
UniProt
Additional ISG15/UCRP Products
Product Documents for Human ISG15/UCRP Antibody
Product Specific Notices for Human ISG15/UCRP Antibody
For research use only
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