Human ITM2C Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4904

R&D Systems, part of Bio-Techne
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AF4904
AF4904-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all ITM2C Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ITM2C
Ala86-Val267
Accession # Q9NQX7

Specificity

Detects human ITM2C in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ITM2A and rhITM2B is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ITM2C Antibody

Detection of Human ITM2C antibody by Western Blot.

Detection of Human ITM2C by Western Blot.

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITM2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2C at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human ITM2C Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: JEG-3 human epithelial choriocarcinoma cell line and human brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITM2C

ITM2C (Integral membrane protein 2C; also BRI3 and cerebral protein 14) is a 36‑38 kDa member of the BRI3 family of proteins. It is found on neurons and appears to interact with APP, where ITM2C binding blocks proteolytic processing of APP, plus SCG10, where ITM2C binding blocks the microtubule-destabilizing activity of SCG10, thus inhibiting neurite outgrowth. Human ITM2C is a 267 amino acid (aa) type II transmembrane glycoprotein. Proteolytic cleavage by furin at Arg242Gly243 may create a truncated 34‑35 kDa ITM2C plus a C-terminal 4 kDa active peptide (aa 243‑267). The ECD (aa 76‑267) contains one BRICHOS domain (aa 136‑230). ITM2C may exist as a dimer. There are two splice variants, one that shows a deletion of aa 41‑87, and a second that shows a deletion of aa 151‑187. Over aa 86‑267, human ITM2C shares 96% aa identity with mouse ITM2C.

Long Name

Integral Membrane Protein 2C

Alternate Names

BRI3, BRICD2C, Cerebral protein 14

Entrez Gene IDs

81618 (Human); 64294 (Mouse); 301575 (Rat)

Gene Symbol

ITM2C

UniProt

Q9NQX7 (Human)

Additional ITM2C Products

Product Documents for Human ITM2C Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ITM2C Antibody

For research use only

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