Human JAM-A Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1103S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 654806
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-A
Ser28-Ala242 (predicted)
Accession # Q9Y624
Ser28-Ala242 (predicted)
Accession # Q9Y624
Specificity
Detects human JAM-A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) JAM-B, recombinant mouse (rm) JAM-A, or rmJAM-4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human JAM-A Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: JAM-A
The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1 - 4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig-like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM-A-LFA-1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).
References
- Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
- Aurand-Lions, M. et al. (2001) Blood 98:3699.
- Sobocka, M.B. et al. (2000) Blood 95:2600.
- Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
- Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
- Ostermann, G. et al. (2002) Nature Immunol. 3:151.
- Barton, E.S. et al. (2001) Cell 104:441.
Long Name
Junctional Adhesion Molecule A
Alternate Names
CD321, F11R, JAMA, PAM-1
Gene Symbol
F11R
UniProt
Additional JAM-A Products
Product Documents for Human JAM-A Alexa Fluor® 750-conjugated Antibody
Product Specific Notices for Human JAM-A Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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