Human KA2/GRIK5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11334
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1060109
Product Specifications
Immunogen
Synthetic peptide sequence
Accession # Q16478
Accession # Q16478
Specificity
Detects human KA2/GRIK5 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human KA2/GRIK5 Antibody
Detection of KA2/GRIK5 in Human Hippocampus.
KA2/GRIK5 was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human KA2/GRIK5 Monoclonal Antibody (Catalog # MAB11334) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human KA2/GRIK5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KA2/GRIK5
References
- Entrez Gene: GRIK5 glutamate receptor, ionotropic, kainate 5.
- Szpirer C, Molne M, Antonacci R, Jenkins NA, Finelli P, Szpirer J, Riviere M, Gilbert DJ, Copeland NG, et al. "The Genese Encoding the Glutamate Receptor Subunits Ka1 and KA2 (GRIK4 and GRIK5) are located on separate chromosomes in Human, Mouse and Rat". Proc Natl Acad Sci U.S.A. 1995 Jan; 91(25):11849.
- Contractor A, Mulle C, Swanson GT. "Kainate Receptors Coming of Age: Milestones of Two Decades of Research". Trends in Neurosciences. 2011 Mar; 34(3):154.
- Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.
Long Name
Glutamate Receptor KA2
Alternate Names
EAA2, GluK5, GRIK2, GRIK5
Gene Symbol
GRIK5
UniProt
Additional KA2/GRIK5 Products
Product Documents for Human KA2/GRIK5 Antibody
Product Specific Notices for Human KA2/GRIK5 Antibody
For research use only
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