Human Kallikrein 11 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1595
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Ile54-Asn282
Accession # Q9UBX7
Ile54-Asn282
Accession # Q9UBX7
Specificity
Detects human Kallikrein 11 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 5, and rhKallikrein 9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Kallikrein 11 Antibody
Kallikrein 11 in Human Prostate.
Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Kallikrein 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1595) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Kallikrein 11 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013), and immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013), and immersion fixed paraffin-embedded sections of human prostate
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 11 (Catalog # 1595-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 11 (Catalog # 1595-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Kallikrein 11 (Catalog # 1595-SE)
Sample: Recombinant Human Kallikrein 11 (Catalog # 1595-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using AF1595 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 11
As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.
References
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225.
- Yousef, G.M. et al. (2000) Genomics 63:88.
- Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
- Gan, L. et al. (2000) Gene 257:119.
- Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
- Nakamura, T. et al. (2003) Urology 61:1042.
- Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.
Alternate Names
KLK11
Entrez Gene IDs
11012 (Human)
Gene Symbol
KLK11
UniProt
Additional Kallikrein 11 Products
Product Documents for Human Kallikrein 11 Antibody
Product Specific Notices for Human Kallikrein 11 Antibody
For research use only
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