Human Kallikrein 12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB40341
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Neutralization, Western Blot
Cited:
Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 364932
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
Ala18-Asn248
Accession # Q9UKR0
Ala18-Asn248
Accession # Q9UKR0
Specificity
Detects human Kallikrein 12 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh)
Kallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, rhKallikrein beta1, rhHGF Activator, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin or rhuPA-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Kallikrein 12 Antibody
Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody.
The cleavage of Boc-VPR-AMC (0.1 mM, Catalog # ES011) by Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) is measured after preincubation with increasing concentrations of Human Kallikrein 12 Monoclonal Antibody (Catalog # MAB40341). The ND50 is typically 0.1 µg/mL.Applications for Human Kallikrein 12 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 12 (Catalog # 3095-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 12 (Catalog # 3095-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Kallikrein 12 (Catalog # 3095-SE) under non-reducing conditions only
Sample: Recombinant Human Kallikrein 12 (Catalog # 3095-SE) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 12
Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family (1). It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat.
References
- Yousef, G.M. et al. (2000) Genomics. 69:331.
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Alternate Names
KLK-L5, KLK12
Entrez Gene IDs
43849 (Human)
Gene Symbol
KLK12
UniProt
Additional Kallikrein 12 Products
Product Documents for Human Kallikrein 12 Antibody
Product Specific Notices for Human Kallikrein 12 Antibody
For research use only
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