Human Kallikrein 2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4104

R&D Systems, part of Bio-Techne
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MAB4104
MAB4104-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Neutralization, Western Blot

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 426723

View all Kallikrein 2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 2
Ile25-Pro261
Accession # P20151

Specificity

Detects human Kallikrein 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or B1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Kallikrein 2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 2 (Catalog # 4104-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Kallikrein 2 (Catalog # 4104-SE).

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 2 (2 µg/mL, Catalog # 4104-SE) cleavage of the fluorogenic peptide substrate PFR-AMC (0.1 mM). The Neutralization Dose (ND50) is typically 3.4 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 2

Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland (1). It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator (2). KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (3‑5). KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer (6).

References

  1. Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
  2. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  3. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  4. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  5. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  6. Sardana, G. et al. (2008) Clin. Chem. 54:1951.

Alternate Names

Klk1c2, KLK2, KLK2A2, Ton

Entrez Gene IDs

3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)

Gene Symbol

KLK2

UniProt

P20151

Additional Kallikrein 2 Products

Product Documents for Human Kallikrein 2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 2 Antibody

For research use only

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