Human Kallikrein 7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB26242
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 333925
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
Glu23-His252
Accession # P49862
Glu23-His252
Accession # P49862
Specificity
Detects human Kallikrein 7 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Kallikrein 7 Antibody
Human Kallikrein 7 ELISA Standard Curve.
Recombinant Human Kallikrein 7 protein was serially diluted 2-fold and captured by Mouse Anti-Human Kallikrein 7 Monoclonal Antibody (MAB26241) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Kallikrein 7 Monoclonal Antibody (Catalog # MAB26242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Kallikrein 7 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when
paired with human anti Kallikrein 7 Monoclonal Antibody (Catalog # MAB26241).This
product is intended for assay development on various assay platforms requiring
antibody pairs.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 7
Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length Kallikrein 7 consists of 253 amino acids (aa), with a signal peptide (aa 1-22), short pro peptide (aa 23-29) and mature chain (aa 30-252) (1). Predominantly expressed in the skin, a major physiological function of Kallikrein 7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for Kallikrein 7 is Kallikrein 5, another member of the human tissue Kallikrein family. Along with Kallikrein 14, these three Kallikreins form a proteolytic cascade in the stratum corneum (5).
References
- Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
- Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
- Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
- Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
- Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
Alternate Names
KLK7
Gene Symbol
KLK7
UniProt
Additional Kallikrein 7 Products
Product Documents for Human Kallikrein 7 Antibody
Product Specific Notices for Human Kallikrein 7 Antibody
For research use only
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