Human Kallikrein 8/Neuropsin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1719
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 189004
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin
Gln29-Gly260 (predicted)
Accession # O60259
Gln29-Gly260 (predicted)
Accession # O60259
Specificity
Detects human Kallikrein 8/Neuropsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein 3, 5, 11, or rhHGFA is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Kallikrein 8/Neuropsin Antibody
Kallikrein 8/Neuropsin in Human Brain.
Kallikrein 8/Neuropsin was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Kallikrein 8/Neuropsin in SH-SY5Y Human Cell Line.
Kallikrein 8/Neuropsin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Kallikrein 8/Neuropsin Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Western Blot
1 µg/mL
Sample: Recombinant Human Kallikrein 8/Neuropsin (Catalog # 2025-SE)
Sample: Recombinant Human Kallikrein 8/Neuropsin (Catalog # 2025-SE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 8/Neuropsin
(aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8).
References
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
- Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
- Davies, B. et al. (2001) J. Neurosci. 21:6993.
- Kishi, T. et al. (2003) Cancer Res. 63:2771.
- Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
- Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
- Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.
Alternate Names
KLK8, Neuropsin, Ovasin, PRSS19
Entrez Gene IDs
11202 (Human)
Gene Symbol
KLK8
UniProt
Additional Kallikrein 8/Neuropsin Products
Product Documents for Human Kallikrein 8/Neuropsin Antibody
Product Specific Notices for Human Kallikrein 8/Neuropsin Antibody
For research use only
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