Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844F) is recommended.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KIR2DS1/CD158h Antibody

Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) from different donors (A and B) were stained with Rabbit Anti-Human KIR2DS1/CD158h Monoclonal Antibody (Catalog # MAB8887) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1844F). Cells were gated on CD56 with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P, not shown).

Applications for Human KIR2DS1/CD158h Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DS1/CD158h

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56^dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1

Alternate Names

CD158h, EB6ActI

Entrez Gene IDs

3806 (Human)

Gene Symbol

KIR2DS1

UniProt

Q14954

Additional KIR2DS1/CD158h Products

Product Documents for Human KIR2DS1/CD158h Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KIR2DS1/CD158h Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KIR2DS1/CD158h Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8887

Key Product Details

Species Reactivity

Validated:

Cited:

Applications