KLF12 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human KLF12 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB9097) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry.

MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Monoclonal Antibody (Catalog # MAB9097, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human KLF12 Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: MG‑63 human osteosarcoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF12

Krueppel-like factor 12 is a protein that in humans is encoded by the KLF12 gene. The protein encoded by this gene confers strong transcriptional repression to the AP-2-alpha gene, a developmentally-regulated transcription factor active during vertebrate development and carcinogenesis. KLF12 has been reported as a novel tumor-suppressor gene whose loss of function is associated with anoikis resistance. Three isoforms have been found for this gene.

Long Name

Kruppel-Like Factor 12

Alternate Names

AP2REP, HSPC122

Entrez Gene IDs

11278 (Human); 16597 (Mouse); 306110 (Rat)

Gene Symbol

KLF12

UniProt

Q9Y4X4

Additional KLF12 Products

Product Documents for Human KLF12 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KLF12 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KLF12 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9097

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all KLF12 Antibodies »

Product Summary for Human KLF12 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human KLF12 Antibody

KLF12 in HEK293 Human Cell Line.