Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross-reactivity with rhKLF1, 4, 5, 6, 10, 12, 17, recombinant mouse KLF4 or 15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human KLF2 Antibody

KLF2 in BG01V Human Cells.

KLF2 was detected in immersion fixed BG01V human embryonic stem cells using Human KLF2 Monoclonal Antibody (Catalog # MAB5466) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of KLF2 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 Monoclonal Antibody (Catalog # MAB5466, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human KLF2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: BG01V human embryonic stem cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF2

KLF2 (Kruppel-like factor 2; also LKLF) is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family. KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells. It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length. It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354). There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.

Long Name

Kruppel-Like Factor 2

Alternate Names

LKLF

Entrez Gene IDs

10365 (Human); 16598 (Mouse); 306330 (Rat)

Gene Symbol

KLF2

UniProt

Q9Y5W3 (Human)

Additional KLF2 Products

Product Documents for Human KLF2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KLF2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KLF2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5466

Key Product Details

Species Reactivity

Validated:

Cited:

Applications