Human Ku70/XRCC6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5597
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Ku70
Asn405-Lys556
Accession # P12956
Asn405-Lys556
Accession # P12956
Specificity
Detects endogenous human Ku70 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Ku70/XRCC6 Antibody
Detection of Human Ku70/XRCC6 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Ku70/XRCC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Ku70/XRCC6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Ku70/XRCC6 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and HEK293 human embryonic kidney cell line
Sample: Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ku70/XRCC6
Ku70 (Lupus Ku autoantigen protein 70; also ATP-dependent DNA helicase 2 subunit 1) is a ubiquitous 70‑75 kDa nuclear protein that serves as a component of the DNA-dependent protein kinase complex DNA-PK. In particular, it heterodimerizes with Ku80 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku70 is 609 amino acids (aa) in length. It contains a Ku core domain (aa 266‑529), a DNA binding motif (aa 268‑274), an NLS (aa 539‑556) and a C-terminal SAP domain (aa 573‑607). There is one potential splice form that shows a deletion of aa 66‑106. Over aa 405‑556, Ku70 shares 83% aa identity with mouse Ku70.
Long Name
Lupus Ku Autoantigen Protein 70
Alternate Names
CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6
Entrez Gene IDs
2547 (Human)
Gene Symbol
XRCC6
UniProt
Additional Ku70/XRCC6 Products
Product Documents for Human Ku70/XRCC6 Antibody
Product Specific Notices for Human Ku70/XRCC6 Antibody
For research use only
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