Detects human LAIR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAIR1, recombinant mouse (rm) LAIR1 or rmLAIR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human LAIR2 Antibody

Detection of LAIR2 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human LAIR2 Monoclonal Antibody (Catalog # MAB2665, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human LAIR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human LAIR2 (Catalog # 2665-LR)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2665 in the following applications:

ELISA (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains (1‑3). Although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR2 is truncated at the C terminus, but retains the entire Ig domain (1‑3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

References

Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
Meyaard, L. et al. (1999) J. Immunol. 162:5800.
Meyaard, L. et al. (1997) Immunity 7:283.
Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

Long Name

Leukocyte-associated Ig-like Receptor 2

Alternate Names

CD306

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Gene Symbol

LAIR2

UniProt

Q6ISS4

Additional LAIR2 Products

Product Documents for Human LAIR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LAIR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LAIR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2665

Key Product Details

Species Reactivity

Validated:

Cited:

Applications