Human Latrophilin 3/LPHN3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5916

R&D Systems, part of Bio-Techne
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AF5916
AF5916-SP
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BAF5916
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Latrophilin 3/LPHN3 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Latrophilin 3/LPHN3
Phe20-Asn813
Accession # NP_056051

Specificity

Detects human Latrophilin 3/LPHN3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Latrophilin 3/LPHN3 Antibody

Detection of Human Latrophilin 3/LPHN3 antibody by Western Blot.

Detection of Human Latrophilin 3/LPHN3 by Western Blot.

Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Latrophilin 3/ LPHN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Latrophilin 3/ LPHN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Latrophilin 3/LPHN3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human cerebellum tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latrophilin 3/LPHN3

LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a predicted 162 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to the brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human LPHN3 is a 7‑TM glycoprotein that is 1428 amino acids (aa) in length. Posttranslational processing cleaves the molecule into a 120 kDa ECD (aa 20‑841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842‑1447). The ECD is modular, and contains a SUEL-like lectin domain (aa 35‑124), an Olf region (aa 134‑393) and GPS domain (aa 802‑853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127‑131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668‑1447. Over aa 20‑813, human LPHN3 shares 98% aa identity with mouse LPHN3.

Alternate Names

CIRL3, LEC3, Lectomedin-3, LPHN3

Entrez Gene IDs

23284 (Human); 319387 (Mouse); 170641 (Rat)

Gene Symbol

ADGRL3

UniProt

NP_056051

Additional Latrophilin 3/LPHN3 Products

Product Documents for Human Latrophilin 3/LPHN3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Latrophilin 3/LPHN3 Antibody

For research use only

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