Human LEDGF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3468
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LEDGF
Met1-Asn530
Accession # O75475
Met1-Asn530
Accession # O75475
Specificity
Detects human LEDGF in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human LEDGF Antibody
Detection of Human LEDGF by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human CD4+T cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LEDGF in HeLa Human Cell Line.
LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human LEDGF by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LEDGF p75 at approximately 99 kDa (as indicated) using 5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human LEDGF Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Knockout Validated
LEDGF
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
LEDGF knockout HeLa cell line.
Simple Western
5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and human CD4+ T cells
Sample: Daudi human Burkitt's lymphoma cell line and human CD4+ T cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LEDGF
Long Name
Lens Epithelium-derived Growth Factor
Alternate Names
CLL-associated antigen KW-7, DFS 70, PSIP1, Transcriptional coactivator p75/p52
Gene Symbol
PSIP1
UniProt
Additional LEDGF Products
Product Documents for Human LEDGF Antibody
Product Specific Notices for Human LEDGF Antibody
For research use only
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