HEK293 human embryonic kidney cell line transfected with (A) human Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB80781) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human Lgr5/GPR49 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Lgr5/GPR49 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr5/GPR49

GPR49 (G-protein-coupled receptor 49), also called LGR5 (leucine-rich repeat GPR 5) is a seven-transmembrane glycoprotein receptor that negatively regulates of Wnt signaling in the developing intestine. Expression of GPR49 is upregulated in intestinal stem cells and intestinal cancer stem cells and promotes carcinogenesis. GPR49 cDNA encodes 907 amino acids (aa), including a long N-terminal extracellular domain (aa 22-561) with 16 LRR domains. Human GPR49 shares 90% aa sequence identity with mouse and rat GPR45 within aa 22-561.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

O75473 (Human)

Additional Lgr5/GPR49 Products

Product Documents for Human Lgr5/GPR49 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Lgr5/GPR49 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Lgr5/GPR49 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB80781

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Lgr5/GPR49 Antibody

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry.