Human CD3+ T cells treated with 1 ng/mL PMA and 1 µg/mL Calcium Ionomycin for 72 hours were stained with (A) Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6643) or (B) isotype control antibody (Catalog # MAB1050) followed by anti-Rabbit IgG APC-conjugated secondary antibody (Catalog # F0111) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Cell Proliferation Induced by LIGHT/TNFSF14 and Neutralization by Human LIGHT/TNFSF14 Antibody.

Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human LIGHT/TNFSF14 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6643). The ND₅₀ is typically 6-48 ng/mL.

Applications for Human LIGHT/TNFSF14 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human CD3+ T cells treated with PMA and Calcium Ionomycin

Neutralization

Measured by its ability to neutralize LIGHT/TNFSF14-induced proliferation in the HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND₅₀) is typically 6-48 ng/mL in the presence of 20 ng/mL Recombinant Human LIGHT/TNFSF14.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT betaR) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

References

Mauri, D.N. et al. (1998) Immunity 8:21.
Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human); 50930 (Mouse)

Gene Symbol

TNFSF14

UniProt

O43557

Additional LIGHT/TNFSF14 Products

Product Documents for Human LIGHT/TNFSF14 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LIGHT/TNFSF14 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry