Human LILRA1/CD85i/LIR-6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30852
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 586439
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6
Pro17-Asn461
Accession # O75019
Pro17-Asn461
Accession # O75019
Specificity
Detects human LILRA1/CD85i/LIR-6 in direct ELISAs. In direct ELISAs, no cross-reactivity with human LILRA2, LILRA3, LILRA4, LILRA5, LILRA6, LILRB2 and LILRB3 was observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human LILRA1/CD85i/LIR-6 Antibody
LILRA1/CD85i/LIR-6 in THP-1 Human Cell Line and PBMCs.
LILRA1/CD85i/LIR-6 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human LILRA1/CD85i/LIR-6 Monoclonal Antibody (Catalog # MAB30852) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human LILRA1/CD85i/LIR-6 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LILRA1/CD85i/LIR-6
Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged aa within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.
Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
Alternate Names
CD85i, LIR-6, LIR6
Entrez Gene IDs
11024 (Human)
Gene Symbol
LILRA1
UniProt
Additional LILRA1/CD85i/LIR-6 Products
Product Documents for Human LILRA1/CD85i/LIR-6 Antibody
Product Specific Notices for Human LILRA1/CD85i/LIR-6 Antibody
For research use only
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