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Human LILRB5/CD85c/LIR-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3065

R&D Systems, part of Bio-Techne
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MAB3065
MAB3065-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 395239

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8 isoform 1
Arg18-His456
Accession # O75023

Specificity

Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, 2, 3, 4, 5, rhLIR-6, or rhILT11/LILRA5 is observed. In Western blots, less than 5% cross-reactivity with rhILT2, 3, 5, rhLIR-6 or rhILT11/LILRA5 is observed, and no cross-reactivity with rhILT1, 4, or 6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human LILRB5/CD85c/LIR-8 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human LILRB5/CD85c/LIR-8

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

Human LIR-8 (leukocyte immunoglobulin-like receptor 8; also CD85c and LILRB5) is a 567 amino acid (aa) member of the LIR family of receptors. It is a type I transmembrane protein that is expressed on NK cells. LIR-8 has a 435 aa extracellular domain with four C2-type Ig-like domains (aa 24‑458), and a 111 aa cytoplasmic tail that contains two ITIM motifs. Phosphorylation of Tyr in an ITIM motif recruits SHP-1, which is subsequently activated and impacts related signaling pathways. The ligand for, and function of, LIR-8 are unreported. LIR-8 has two potential splice forms. One shows a 53 aa cytoplasmic insert between Arg542 and Ala543. The second shows a deletion of 100 aa between Gly119 and Gly219 that impacts the second C2-type Ig-like domain. There is no rodent ortholog.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Alternate Names

CD85c, LIR-8, LIR8

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Gene Symbol

LILRB5

UniProt

Additional LILRB5/CD85c/LIR-8 Products

Product Documents for Human LILRB5/CD85c/LIR-8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LILRB5/CD85c/LIR-8 Antibody

For research use only

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