Human LIM1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2725
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 320416
Product Specifications
Immunogen
E. coli-derived recombinant human LIM1
Leu265-Trp406
Accession # P48742
Leu265-Trp406
Accession # P48742
Specificity
Detects human LIM1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human LIM1 Antibody
LIM1 in Human Brain (Cerebellum).
LIM1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human LIM1 Monoclonal Antibody (Catalog # MAB2725) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human LIM1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Western Blot
1 µg/mL
Sample: Recombinant Human LIM1
Sample: Recombinant Human LIM1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB2725 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIM1
LIM homeobox 1 gene product (LIM1), also known as LHX-1, is a homeobox nuclear transcription factor expressed in human brain, thymus, tonsil and many myeloid leukemias. The 406 amino acid human LIM1 contains a LIM domain with twin zinc-binding sequences and a homeobox DNA-binding domain. The C-terminal sequence used as an immunogen is virtually identical between human and mouse LIM1. A truncated human isoform does not include this sequence. LIM1 activity is controlled by interaction with the ubiquitous nuclear adaptor protein Ldb-1 (Lim-domain-binding protein-1) which binds the LIM domain and mediates dimerization.
Additional LIM1 Products
Product Documents for Human LIM1 Antibody
Product Specific Notices for Human LIM1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...