Human LINGO-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3086

R&D Systems, part of Bio-Techne
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AF3086
AF3086-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all LINGO-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LINGO-1
Thr40-Thr556
Accession # NP_116197

Specificity

Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human LINGO-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LINGO-1 Antibody

LINGO-1 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr).

LINGO‑1 in Mouse Spinal Cord.

LINGO-1 was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human LINGO-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3086) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human LINGO-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1.7-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse and rat spinal cord

Western Blot

0.1 µg/mL
Sample: Recombinant Human LINGO-1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LINGO-1

LINGO-1 (LRRN6A) is a 614 aa transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa identity between human, mouse and rat.

Long Name

Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1

Alternate Names

LERN1, LINGO1, LRRN6A

Entrez Gene IDs

84894 (Human)

Gene Symbol

LINGO1

UniProt

NP_116197

Additional LINGO-1 Products

Product Documents for Human LINGO-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LINGO-1 Antibody

For research use only

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