Human Lipin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3885
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693
Asp448-Leu574
Accession # Q14693
Specificity
Detects human Lipin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Lipin 1 Antibody
Lipin 1 in Human Adipocytes.
Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Lipin 1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cell-derived adipocytes
Sample: Immersion fixed human mesenchymal stem cell-derived adipocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human Lipin 1
Sample: Recombinant Human Lipin 1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipin 1
Long Name
Phosphatidate Phosphatase LPIN1
Alternate Names
LPIN1
Gene Symbol
LPIN1
UniProt
Additional Lipin 1 Products
Product Documents for Human Lipin 1 Antibody
Product Specific Notices for Human Lipin 1 Antibody
For research use only
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