Human LMO2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2726

R&D Systems, part of Bio-Techne
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BAF2726
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AF2726
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all LMO2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LMO2 isoform 1
Met1-Ile158
Accession # P25791

Specificity

Detects human LMO2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LMO2 Biotinylated Antibody

LMO2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

LMO2 in K562 Human Cell Line.

LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human LMO2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2726) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human LMO2 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human LMO2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Entrez Gene IDs

4005 (Human)

Gene Symbol

LMO2

UniProt

P25791

Additional LMO2 Products

Product Documents for Human LMO2 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LMO2 Biotinylated Antibody

For research use only

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