Human LNK/SH2B3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5888

R&D Systems, part of Bio-Techne
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AF5888
AF5888-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all LNK/SH2B3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LNK/SH2B3
Gln427-Leu575
Accession # Q9UQQ2

Specificity

Detects human LNK/SH2B3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LNK/SH2B3 Antibody

Detection of Human LNK/SH2B3 antibody by Western Blot.

Detection of Human LNK/SH2B3 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH-SY5Y human neuroblastoma cell line, Ramos human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LNK/SH2B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LNK/SH2B3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LNK/SH2B3 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, SH-SY5Y human neuroblastoma cell line, Ramos human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LNK/SH2B3

LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS‑PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24‑81), a PH domain that may interact with membrane lipids (aa 197‑305), and an SH2 domain that binds phosphoTyr (aa 364‑462).

Long Name

Lymphocyte Adapter Protein

Alternate Names

SH2B3

Entrez Gene IDs

10019 (Human); 16923 (Mouse)

Gene Symbol

SH2B3

UniProt

Q9UQQ2 (Human)

Additional LNK/SH2B3 Products

Product Documents for Human LNK/SH2B3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LNK/SH2B3 Antibody

For research use only

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