Human LPAR5/LPA5/GPR92 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10297
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 459812
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human LPAR5/LPA5/GPR92
Accession # Q9H1C0
Accession # Q9H1C0
Specificity
Detects human LPAR5/LPA5/GPR92 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human LPAR5/LPA5/GPR92 Antibody
Detection of LPAR5/LPA5/GPR92 in HEK293 Human Cell Line Transfected with Human LPAR5/LPA5/GPR92 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human LPAR5/LPA5/GPR92 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LPAR5/LPA5/GPR92 Monoclonal Antibody (Catalog # MAB10297) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.LPAR5/LPA5/GPR92 in HT‑29 Human Cell Line.
LPAR5/LPA5/GPR92 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line treated with sodium butyrate (positive stain) and untreated (negative stain) using Mouse Anti-Human LPAR5/LPA5/GPR92 Monoclonal Antibody (Catalog # MAB10297) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.LPAR5/LPA5/GPR92 in Human Colon.
LPAR5/LPA5/GPR92 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human LPAR5/LPA5/GPR92 Monoclonal Antibody (Catalog # MAB10297) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Goblet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human LPAR5/LPA5/GPR92 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/mL
Sample: HEK293 Human Cell Line Transfected with Human LPAR5/LPA5/GPR92 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human LPAR5/LPA5/GPR92 and eGFP
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line treated with sodium butyrate
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line treated with sodium butyrate
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB10297 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LPAR5/LPA5/GPR92
Long Name
Lysophosphatidic Acid Receptor 5
Alternate Names
GPR92, GPR93, KPG_010, LPA5
Gene Symbol
LPAR5
UniProt
Additional LPAR5/LPA5/GPR92 Products
Product Documents for Human LPAR5/LPA5/GPR92 Antibody
Product Specific Notices for Human LPAR5/LPA5/GPR92 Antibody
For research use only
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