Human LRP-1 Cluster II Antibody

Detection of Human LRP-1 Cluster II by Western Blot.

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-1 Cluster II Monoclonal Antibody (Catalog # MAB2368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for LRP-1 Cluster II at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

LRP-1 Cluster II in Human Brain (Cortex).

LRP-1 Cluster II was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human LRP-1 Cluster II Monoclonal Antibody (Catalog # MAB2368) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.