Human Lymphotoxin-alpha /TNF-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB621
Discontinued Product
MAB621 has been discontinued.
An alternative/replacement product is available:
MAB621R. View all Lymphotoxin-alpha/TNF-beta products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
ELISpot Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 5807
Product Specifications
Immunogen
E. coli-derived recombinant human Lymphotoxin‑ alpha/TNF‑ beta
Specificity
Detects human Lymphotoxin‑ alpha/TNF‑ beta in ELISAs. In sandwich immunoassays, approximately 10% cross‑reactivity with recombinant mouse TNF‑ beta is observed and no cross-reactivity with bovine FGF acidic, recombinant human (rh) GM‑CSF, rhIL‑1 alpha, rhIL‑1 beta, rhIL‑2, rhIL‑3, rhIL‑4, rhIL‑6, rhIL‑8, rhTNF‑ alpha, rhPDGF, rpPDGF, rhTGF‑ beta1, recombinant porcine (rp) TGF‑ beta1, rpTGF‑ beta1.2, or rpTGF‑ beta2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Lymphotoxin-alpha /TNF-beta Antibody
Cytotoxicity Induced by Lymphotoxin‑ alpha/TNF‑ beta and Neutral-ization by Human Lymphotoxin‑ alpha/TNF‑ beta Antibody.
Recombinant Human Lymphotoxin-a/TNF-beta (Catalog # 211-TBB) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human Lymphotoxin-a/TNF-beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Lymphotoxin-a/TNF-beta Monoclonal Antibody (Catalog # MAB621). The ND50 is typically 0.03-0.09 µg/mL in the presence of the metabolic inhibitor actinomycin D.Applications for Human Lymphotoxin-alpha /TNF-beta Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Lymphotoxin‑ alpha/TNF‑ beta-induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha/TNF‑ beta and actinomycin D.
Human Lymphotoxin-alpha /TNF-beta Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin-alpha/TNF-beta
Tumor necrosis factor beta (TNF-beta), also known as lymphotoxin-alpha (LT-alpha), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.
TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.
Long Name
Tumor Necrosis Factor beta
Alternate Names
LTA, Lymphotoxin alpha, Lymphotoxinalpha, TNF-beta, tnfb, TNFSF1, TNFSF1B
Gene Symbol
LTA
Additional Lymphotoxin-alpha/TNF-beta Products
Product Documents for Human Lymphotoxin-alpha /TNF-beta Antibody
Product Specific Notices for Human Lymphotoxin-alpha /TNF-beta Antibody
For research use only
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