Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2639

R&D Systems, part of Bio-Techne
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MAB2639
MAB2639-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 262418

View all Lysyl Oxidase Homolog 2/LOXL2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lysyl Oxidase Homolog 2/LOXL2
Gln26-Gln744 (predicted)

Specificity

Detects human Lysyl Oxidase Homolog 2/LOXL2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Lysyl Oxidase Homolog 2/LOXL2 (Catalog # 2639-AO), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Lysyl Oxidase Homolog 2/LOXL2 under non-reducing conditions only (Catalog # 2639-AO)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysyl Oxidase Homolog 2/LOXL2

Lysyl Oxidase Homolog 2 (lysyl oxidase-like protein 2, LOXL2) is a member of lysyl oxidase-like (LOXL) gene family which includes LOXL1 through LOXL4. These enzymes are secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes (1). The N-terminal region of LOXL2 contains four scavenger receptor cysteine-rich (SRCR) domains, and the C-terminal region is a catalytic domain similar to other lysyl oxidases (1). The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone co-factor (2). Although some of the LOXL enzymes are known to cross-link collagen and elastin substrates, such a function has yet to be characterized for LOXL2. It has been shown that LOXL2 promotes cell migration and tumor cell invasiveness (3, 4). Elevated expression of LOXL2 is also associated with cancer progression in various tumors and carcinoma cell lines, which makes it a potential marker for prognosis of cancer (5). LOXL2 is expressed in many tissues, with elevated levels in reproductive tissues such as placenta, uterus, and prostate (6).

References

  1. Csiszar, H. (2001) Prog. Nucleic Acid Res. Mol. Biol. 70:1.
  2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
  3. Akiri, G. et al. (2003) Cancer Res. 63:1657.
  4. Hollosi, P. et al. (2009) Int. J. Cancer. 125:318.
  5. Peinado, H. et al. (2008) Cancer Res. 68:4541.
  6. Jourdan-Le Saux C. et al. (1999) J. Biol. Chem. 274:12939.

Alternate Names

LOL2, LOXL2

Entrez Gene IDs

4017 (Human); 94352 (Mouse); 102138804 (Cynomolgus Monkey)

Gene Symbol

LOXL2

Additional Lysyl Oxidase Homolog 2/LOXL2 Products

Product Documents for Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

For research use only

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