Human Lysyl Oxidase Homolog 3/LOXL3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6069

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB6069-100
MAB6069-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1013328

View all Lysyl Oxidase Homolog 3/LOXL3 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Lysyl Oxidase Homolog 3/LOXL3 protein
Met1-Ile753
Accession # P58215

Specificity

Detects human Lysyl Oxidase Homolog 3/LOXL3 protein in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Lysyl Oxidase Homolog 3/LOXL3 Antibody

Human Lysyl Oxidase Homolog 3/LOXL3 Antibody in ELISA Standard Curve.

Human Lysyl Oxidase Homolog 3/LOXL3 ELISA Standard Curve.

Recombinant Human Lysyl Oxidase Homolog 3/LOXL3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB60691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB6069) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Lysyl Oxidase Homolog 3/LOXL3 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB60691).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysyl Oxidase Homolog 3/LOXL3

Lysyl oxidase-like protein 3 (LOXL3) is a secreted copper amine oxidase. The N-terminal region of LOXL3 contains four scavenger receptor cysteine-rich (SRCR) domains. The C-terminal region consists of a catalytic domain similar to other lysyl oxidases and a cytokine receptor-like domain (1). The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone cofactor (2). LOXL3 is expressed primarily in epidermis, kidney, aorta, and chondrocytes (3). The enzyme is expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3 oxidizes the lysine epsilon-amino groups of a number of collagen substrates, and is also active against other primary amines (4). However, LOXL3 does not require proteolytic processing to be activated.

References

  1. Csiszar, K. (2001) Prog. Nucl. Acid Res. Mol. Biol. 70:1.
  2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
  3. Molnar, J. et al. (2003) Biochim. Biophys. Acta 1647:220.
  4. Lee, J. and Y. Kim (2006) J. Biol. Chem. 281:37282.

Alternate Names

LOL3, LOXL3

Entrez Gene IDs

84695 (Human); 16950 (Mouse)

Gene Symbol

LOXL3

UniProt

P58215

Additional Lysyl Oxidase Homolog 3/LOXL3 Products

Product Documents for Human Lysyl Oxidase Homolog 3/LOXL3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lysyl Oxidase Homolog 3/LOXL3 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.