Human MBD3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3839
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MBD3 isoform 2
Met1-Val259
Accession # AAH43619
Met1-Val259
Accession # AAH43619
Specificity
Detects human MBD3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human MBD3 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MBD3
Sample: Recombinant Human MBD3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MBD3
MBD3 (methyl-CpG-binding domain protein # 3) is a 38‑40 kDa member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD2. Human MBD3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa’s 1‑72) and a C-terminal coiled-coil region (aa’s 216‑245) that may be involved in protein-protein interaction. Human MBD3 shares 95% aa identity with mouse, rat, and canine MBD3, with differences essentially limited to the C‑terminal 25 amino acids.
Long Name
Methyl-CpG Binding Domain Protein 3
Alternate Names
methyl-CpG binding domain protein 3, methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3
Gene Symbol
MBD3
UniProt
Additional MBD3 Products
Product Documents for Human MBD3 Antibody
Product Specific Notices for Human MBD3 Antibody
For research use only
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