Human MCAM/CD146 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB932F

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 128018

View all MCAM/CD146 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
Val24-Gly559
Accession # AAA20824

Specificity

Detects human MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm)OCAM, or rmMAdCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MCAM/CD146 Fluorescein-conjugated Antibody

Detection of MCAM/CD146 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB932F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MCAM/CD146 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

MCAM, also known as CD146 and MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

AAA20824

Additional MCAM/CD146 Products

Product Documents for Human MCAM/CD146 Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MCAM/CD146 Fluorescein-conjugated Antibody

For research use only

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