Detects human MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm)OCAM, or rmMAdCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human MCAM/CD146 Fluorescein-conjugated Antibody

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB932F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MCAM/CD146 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

MCAM, also known as CD146 and MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

AAA20824

Additional MCAM/CD146 Products

Product Documents for Human MCAM/CD146 Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MCAM/CD146 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human MCAM/CD146 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB932F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications