Human MCEMP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4994
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MCEMP1
Val106-Gln187
Accession # Q8IX19
Val106-Gln187
Accession # Q8IX19
Specificity
Detects human MCEMP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human MCEMP1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood and THP-1 human acute monocytic leukemia cell line
Sample: Human whole blood and THP-1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human MCEMP1
Sample: Recombinant Human MCEMP1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCEMP1
MCEMP1 (mast cell-expressed membrane protein 1; also C19orf59) is a 22 kDa mast cell specific protein expressed in lung. Human MCEMP1 is 187 amino acids (aa) in length. It is a type II transmembrane protein that contains an 85 aa N-terminal cytoplasmic region and an 81 aa C-terminal extracellular domain (aa 107‑187). There is one potential splice variant that shows a 22 aa substitution for the C-terminal five amino acids. Over aa 106‑187, human MCEMP1 shares only 52% aa identity with the presumed mouse MCEMP1 ortholog.
Long Name
Mast Cell-expressed Membrane Protein 1
Alternate Names
C19orf59
Gene Symbol
MCEMP1
UniProt
Additional MCEMP1 Products
Product Documents for Human MCEMP1 Antibody
Product Specific Notices for Human MCEMP1 Antibody
For research use only
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