Human MDL-1/CLEC5A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2384
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
Tyr26-Lys188
Accession # Q9NY25
Tyr26-Lys188
Accession # Q9NY25
Specificity
Detects human MDL‑1/CLEC5A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse MDL-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MDL-1/CLEC5A Antibody
Detection of Human MDL‑1/CLEC5A by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MDL-1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2384) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MDL-1/CLEC5A at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MDL‑1/CLEC5A in Human Joint.
MDL-1/CLEC5A was detected in formalin fixed paraffin-embedded sections of human rheumatoid arthritis joint using Goat Anti-Human MDL-1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2384) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of monocyte colony cells in rheumatoid arthritis joint. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MDL-1/CLEC5A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Immunohistochemistry
5-15 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human rheumatoid arthritis joint
Sample: Formalin fixed paraffin-embedded sections of human rheumatoid arthritis joint
Western Blot
1 µg/mL
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MDL-1/CLEC5A
Long Name
Myeloid DAP12-associating Lectin
Alternate Names
CLEC5A, CLECSF5, MDL1
Gene Symbol
CLEC5A
UniProt
Additional MDL-1/CLEC5A Products
Product Documents for Human MDL-1/CLEC5A Antibody
Product Specific Notices for Human MDL-1/CLEC5A Antibody
For research use only
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