Human MEK2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2855
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MEK2
Met1-Val400
Accession # P36507
Met1-Val400
Accession # P36507
Specificity
Detects human MEK2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MEK2 Biotinylated Antibody
Detection of Human MEK2 by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human MEK2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2855) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (10 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MEK2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line
Sample: Ramos human Burkitt's lymphoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEK2
Mitogen-activated protein kinase kinase 2 (MAP2K2), also known as MEK2 and MKK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dual-specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK2 by Raf occurs through phosphorylation at S222 and S226.
Long Name
Mitogen-activated Protein Kinase Kinase 2
Alternate Names
MAP2K2, MKK2
Gene Symbol
MAP2K2
UniProt
Additional MEK2 Products
Product Documents for Human MEK2 Biotinylated Antibody
Product Specific Notices for Human MEK2 Biotinylated Antibody
For research use only
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