Human Menin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6005
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Menin
aa 555-615
Accession # O00255
aa 555-615
Accession # O00255
Specificity
Detects human Menin in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Menin Antibody
Detection of Human Menin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Menin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Menin at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Menin Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Menin
Menin (Multiple endocrine neoplasia type I) is a 75‑78 kDa tumor‑suppressor protein that has no apparent structural relationship to any known protein family. It is ubiquitously expressed and interacts with other molecules, including NF kappaB, FANCD2 and ASK. Menin appears to block gene transcription, promote DNA repair, and inhibit cell proliferation. Human Menin is 615 amino acids (aa) in length. Consistent with its unique molecular taxonomy, it contains no definitive structural module(s). However, it is known to be phosphorylated on Ser399 and Thr599. There are a number of potential splice variants. Individually or in combination, there are deletions of aa 89‑95, 149‑153, 171‑173, 189‑223, and 423‑426. There is also a 50 aa substitution for aa 68‑615, a 66 aa substitution for aa 383‑615, and a two aa substitution for aa 507‑536. Over aa 555‑615, human and mouse Menin are completely identical in aa sequence.
Long Name
Multiple Endocrine Neoplasia I
Alternate Names
MEAI, MEN1
Gene Symbol
MEN1
UniProt
Additional Menin Products
Product Documents for Human Menin Antibody
Product Specific Notices for Human Menin Antibody
For research use only
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