Human MERIT40 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6604
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MERIT40
Asn182-Val329
Accession # Q9NWV8
Asn182-Val329
Accession # Q9NWV8
Specificity
Detects human MERIT40 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human MERIT40 Antibody
Detection of Human MERIT40 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gel was loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Human MERIT40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6604) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MERIT40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MERIT40 in Human Breast Cancer Tissue.
MERIT40 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human MERIT40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6604) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MERIT40 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MERIT40
MERIT40 (MEdiator of Rap80 Interactions and Targeting subunit of 40 kDa; also NBA1 and c19orf62 and HSPC142) is a 40 kDa member of the MERIT40 family of proteins. It is ubiquitously expressed, and participates in the DNA damage response. At sites of double strand breaks, histones are phosphorylated, ubiquitinated, and deubiquitinated, with repairs performed by a sequentially formed complex that contains BRCA1, Rap80 and BRE. MERIT40 apparently serves as a scaffold protein that maintains the integrity of the complex. Human MERIT40 is 329 amino acids (aa) in length. It contains one VWF-A domain (aa 95-298) and six utilized Ser/Thr phosphorylation sites. There are three potential splice variants. One shows a 13 aa substitution for aa 234-329, a second possesses a 105 aa substitution for aa 263‑329, while a third shows a 37 aa substitution for aa 1-115, coupled to a deletion of aa 263-329. Over aa 182-329, human MERIT40 shares 95% aa identity with mouse MERIT40.
Long Name
Mediator of RAP80 Interactions and Targeting Subunit of 40 kDa
Alternate Names
BABAM1, C19orf62, HSPC142, NBA1
Gene Symbol
BABAM1
UniProt
Additional MERIT40 Products
Product Documents for Human MERIT40 Antibody
Product Specific Notices for Human MERIT40 Antibody
For research use only
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